The article describes the mycological examination of the church, built at 1900 in the village Penino. Detailed visual inspection was confirmed by photo fixing, there were taken samples from different constructions, where were a lot of visible efflorescence, scuffs of green colors, noticeable lamination structure of the material, macro fouling of the natural stone with moss and algae. Additionally the results of mycological laboratory analysis of taken samples were presented. According to the received results, that showed the number of colony forming units and the type of microorganism, there were given recommendations how to remove all fixed colonies of microorganisms that ruin the object’s construction.